Under Review

Ultrasound-guided core biopsy of FDG-PET positive supraclavicular fat to investigate and quantify molecular markers of brown adipose tissue

  • Julian Müller
  • Anton Becker
  • Miroslav Balaz
  • Vissarion Efthymiou
  • Claudia Maushart
  • Gani Gashi
  • Edwin terVoert
  • Christian Wolfrum
  • Matthias Betz
  • Irene Burger
Brown fat Brown adipose tissue FDG PET CT Hybrid Imaging Ultrasound Image guided Biopsy Supraclavicular Soft tissue

Cite as:

Julian Müller and Anton Becker and Miroslav Balaz and Vissarion Efthymiou and Claudia Maushart and Gani Gashi and Edwin terVoert and Christian Wolfrum and Matthias Betz and Irene Burger (2019). Ultrasound-guided core biopsy of FDG-PET positive supraclavicular fat to investigate and quantify molecular markers of brown adipose tissue. RESEARCHERS.ONE, https://www.researchers.one/article/2019-10-11.

Abstract:

Purpose

To investigate transcutaneous core-needle biopsy of the supraclavicular fat as a minimally invasive and scar-free method of obtaining brown adipose tissue (BAT) samples.

Material and Methods

In a prospective clinical trial, 16 volunteers underwent biopsy on two separate occasions after FDG-PET had shown active BAT in the supraclavicular fossa with an FDG uptake (SUVmax) > 3 mg/dl. After identifying the ideal location for biopsy on FDG-PET/MRI, ultrasound-guided core-needle biopsy of supraclavicular fat with a 16G needle was performed under local anesthesia and aseptic conditions. Tissue samples were immediately shock-frozen in liquid nitrogen and processed for gene expression analysis of adipose tissue markers. Wounds were checked two weeks after the biopsy.

Results

Tissue sampling was successful in 15 volunteers in both scans and in one very lean volunteer (BMI=19.9 kg/m2) in only one visit, without any reported adverse events. Therefore 31 tissue samples were available for further analysis. Gene expression could be analyzed with high success rate in 30 out of 31 tissue biopsies. The intervention was well tolerated with local anesthetics. None of the volunteers showed any scarring.

Conclusion

Ultrasound-guided core-needle biopsy of FDG-positive supraclavicular fat yields sufficient BAT samples for quantification of molecular markers. It may, however, be limited in extremely lean individuals with very little supraclavicular fat.